GENDER DIFFERENCES IN LEVELS OF STEROID HORMONES IN HEART CELL MITOCHONDRIA AT THE STAGES OF B16/F10 MELANOMA DEVELOPMENT COMBINED WITH CHRONIC NEUROGENIC PAIN

Aim - an analysis of levels steroid hormones in heart cell mitochondria at the stages B16/F10 melanoma development combination with chronic neurogenic pain male and female C57BL/6 mice. The study included mice (n=336). Experimental groups were: intact animals (♂ n=21; ♀ n=21); controls n=21) a model (CNP); comparison group n=63; n=63) melanomas (B16/ F10); main (CNP+B16/F10) transplanted 3 weeks after CNP creation. Levels estradiol (pg/g protein), estrone progesterone (ng/g total testosterone free protein) were determined by ELISA mitochondrial samples. In CNP, changes detected only mice: was reduced 4.6 times, 2.2 1.8 times (p˂0.05), 2.4 compared values. Melanoma growth (3 weeks) females downregulated 1.7 2.1 2.3 males, decreased during same period. presence 1.6 (p˂0.05) control val- ues, while males showed 1.3 1.9 2.7 respectively, as well elevated (p˂0.05). to greater extent weaken due involvement wider spectrum decrease their mitochondria, males. When combined melanoma, more significant hormonal imbalance than females.